Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503061)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDEL1 (RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1.
HEK293T cells transfected with either RC212323 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NDEL1 antibody (TA503061), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-NDEL1 antibody (TA503061), (Red), compared to a nonspecific negative control antibody, (Blue).