ENOA Antibody (N-term) (Cat. #TA324751) western blot analysis in U251, mouse C2C12, mouse NIH/3T3 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENOA antibody detected the ENOA protein (arrow).
IF image of Hela cell stained with ENOA Antibody (N-term) (Cat#TA324751).Hela cells were incubated with ENOA primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue). ENOA immunoreactivity is localized to Cytoplasm and Nucleus significantly.
ENOA Antibody (N-term) (Cat. #TA324751) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.