Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-Nog monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-Nog mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500028)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nog (RC205020, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nog. Positive lysates LY401672 (100ug) and LC401672 (20ug) can be purchased separately from OriGene.
Noggin antibody (4C12) at 1:2000 + Recombinant human Noggin (Cat# RC205020)
Anti-Nog mouse monoclonal antibody (TA500028) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY Nog (RC205020).
Immunofluorescent staining of A549 cells using anti-Nog mouse monoclonal antibody (TA500028).