HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT. Positive lysates LY401860 (100ug) and LC401860 (20ug) can be purchased separately from OriGene.
Anti-NNMT mouse monoclonal antibody (TA502538) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NNMT (RC200641).
Flow cytometric Analysis of Hela cells, using anti-NNMT antibody (TA502538), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NNMT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and NNMT-Knockout HeLa cells (KO, Cat# LC832761) were separated by SDS-PAGE and immunoblotted with anti-NNMT monoclonal antibody TA502538 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502538)
Flow cytometric Analysis of Jurkat cells, using anti-NNMT antibody (TA502538), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells transfected with either RC200641 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NNMT antibody (TA502538), and then analyzed by flow cytometry.