HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NME4 (RC202603, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NME4. Positive lysates LY401559 (100ug) and LC401559 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HEK293T cells (WT, Cat# LC810293T) and NME4-Knockout HEK293T cells (KO, Cat# LC870008) were separated by SDS-PAGE and immunoblotted with anti-NME4 monoclonal antibody TA501115 (1:500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Anti-NME4 mouse monoclonal antibody (TA501115) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NME4 (RC202603).
HEK293T cells transfected with either pCMV6-ENTRY NME4 (RC202603) (Red) or empty vector control plasmid (Blue) were immunostained with anti-NME4 mouse monoclonal (TA501115), and then analyzed by flow cytometry.