The store will not work correctly in the case when cookies are disabled.
Menu
  •  
  • Call:+44 (0) 1235 828 200
  • orContact Us
  • Call:+44 (0) 1235 828 200
  • orContact Us
  • Call:+41 (0) 91 604 55 22
  • orContact Us
  • Call:+49 (0) 69 779099
  • orContact Us
  • Call:+31 (0) 72 8080244
  • orContact Us
  • Call:+1 (617) 945 5033
  • orContact Us
amsbio_new_logoamsbio_new_logoamsbio_new_logoamsbio_new_logo
  • My Account
    • log in
    • create account
  • My Cart
    My Cart 1
Menu
  • Products
    • Close
    • Biorepository
      • Close
        • DNA
          • Close
          • Tissue-procurement-cta
            We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • RNA
          • Close
          • Tissue-procurement-cta
            We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • Tissues
          • Close
          • Tissue-procurement-cta
            We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • Biofluids
          • Close
          • Tissue-procurement-ctaImage
            We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • Proteins
          • Close
          • Tissue-procurement-ctaImage
            We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • Primary Cells
          • Close
          • Tissue-procurement-ctaImage
            We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
        • FFPE Cell Line Controls
          • Close
          • Tissue-procurement-ctaImage
            We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells.
            Learn More
    • Molecular Biology
      • Close
        • Cloning Vectors
          • Close
          • Product cat CTA2Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • cDNA Clones
          • Close
          • Product cat CTA2Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • RNAi
          • Close
          • Product cat CTA2Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Genome Editing
          • Close
          • Product cat CTA2Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • PCR Reagents
          • Close
          • Product cat CTA2Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Transfection Reagents
          • Close
          • Product cat CTA2Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Nucleic Acid Isolation Kits
          • Close
          • Product cat CTA2Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Molecular Biology Enzymes
          • Close
          • Product cat CTA2Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Magnetic Beads
          • Close
          • Product cat CTA2Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
        • Other
          • Close
          • Product cat CTA2Image
            We provide the most advanced method for gene and shRNA delivery with an extensive collection of viral particles as well as a range of custom services.
            Learn More
    • Antibodies
      • Close
        • Monoclonal
          • Close
          • Product cat CTA3
            Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.
            Learn More
        • Polyclonal
          • Close
          • Product cat CTA3
            Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.
            Learn More
        • Secondary Antibodies
          • Close
          • Product cat CTA3
            Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications.
            Learn More
    • Proteins & Peptides
      • Close
        • Recombinant Proteins
          • Close
          • Product cat CTA4Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Overexpression Lysates
          • Close
          • Product cat CTA4Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Overexpression FFPE Cell Line Sections
          • Close
          • Product cat CTA4Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Mass Spec Standards
          • Close
          • Product cat CTA4Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Cell Line Lysates
          • Close
          • Product cat CTA4Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Purified Natural Proteins
          • Close
          • Product cat CTA4Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Peptides and Blocking Peptides
          • Close
          • Product cat CTA4Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
        • Magnetic Beads for Protein Applications
          • Close
          • Product cat CTA4Image
            ALiCE® is a radically different approach to cell-free expression and a kit unlike any others.
            Learn More
    • Cells & Cell Culture
      • Close
        • Cell Lines
          • Close
          • Product cat CTA5Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • FFPE Cell Line Controls
          • Close
          • Product cat CTA5Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Cell Line Lysates
          • Close
          • Product cat CTA5Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Primary Cells
          • Close
          • Product cat CTA5Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Stem Cells
          • Close
          • Product cat CTA5Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Cell Culture Kits
          • Close
          • Product cat CTA5Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • 3D Cell Culture & Extracellular Matrices
          • Close
          • Product cat CTA5Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Media and Reagents
          • Close
          • Product cat CTA5Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Transfection Reagents
          • Close
          • Product cat CTA5Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
        • Viral Delivery
          • Close
          • Product cat CTA5Image
            The CELLBANKER® series of cryopreservation media allows for the stable long-term storage of any cell type, including sensitive cell lines.
            Learn More
    • Reagents & Labware
      • Close
        • Other Enzymes
          • Close
          • Product cat CTA6Image
            Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.
            Learn More
        • Labware and Equipment
          • Close
          • Product cat CTA6Image
            Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.
            Learn More
        • Reagents & Consumables
          • Close
          • Product cat CTA6Image
            Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.
            Learn More
        • Carbohydrates
          • Close
          • Product cat CTA6Image
            Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.
            Learn More
        • Gold & Silver Coated Substrates
          • Close
          • Product cat CTA6Image
            Prepared from Arthrobacter luteus, Zymolyase has a highly effective lytic activity against yeast cell walls.
            Learn More
    • Kits & Assays
      • Close
        • Protein Kits
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Apoptosis Kits
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • ELISA Kits
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Cell Migration/Invasion Kits
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Enzyme Activity Assays
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Cell Proliferation and Viability Kits
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Stem Cell Kits
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Cell Culture Kits
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Exosome Kits
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Metabolism Kits
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Conjugation Kits
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
        • Other Kits
          • Close
          • Product cat CTA7Image
            AMSBIO offers one of the largest selection of active recombinant enzymes and enzyme activity assays for profiling, inhibitor screening assays, and drug discovery.
            Learn More
    • Bioseparation & Electrophoresis
      • Close
        • Bioseparation Reagents
          • Close
          • Product cat CTA8Image
            We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.
            Learn More
        • Chromatography Media
          • Close
          • Product cat CTA8Image
            We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.
            Learn More
        • Electrophoresis Equipment
          • Close
          • Product cat CTA8Image
            We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.
            Learn More
        • Pre-cast Gels
          • Close
          • Product cat CTA8Image
            We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.
            Learn More
        • Magnetic Beads
          • Close
          • Product cat CTA8Image
            We supply a range of superior magnetic products each uniquely suited to specific protein purification, proteomics and genomics applications.
            Learn More
  • Research Areas
    • Close
    • Cancer Research
    • Cardiovascular
    • Exosomes
    • Immunology
    • Cellular Metabolism
    • Inflammation & Arthritis
    • Neuroscience
    • Glycobiology
    • Stem Cells
    • Epigenetics
    • 3D Cell Culture
    • Cell & Gene Therapy
  • Resources
  • Custom Services
    • Close
    • Protein Expression
      • Close
        • Mammalian Protein Expression
        • Bacterial Protein Expression
        • Baculovirus Protein Expression
        • Cell Free Protein Expression
    • Lentivirus & Adenovirus & AAV
      • Close
        • Lentivirus ORF Expression
        • Lentivirus shRNA Expression
        • AAV
        • Lentivirus microRNA
        • Lentivirus anti miRNA
        • Adenovirus
        • Vesicular Stomatitis Virus (VSV) Packaging –
    • Peptide & Antibody
      • Close
        • Peptide Synthesis
        • Monoclonal Antibody Service
        • Recombinant Monoclonal Antibody Service
        • Polyclonal Antibody Service
        • Phospho Polyclonal Antibody
        • Licensing –
    • Tissue Procurement
    • Histochemistry
    • Stem Cell Differentiation
    • Cell Based Assays
    • Drug Screening Service
    • CAR-T Development –
    • Magnetic Beads
    • Molecular Biology
      • Close
        • CRISPR Gene Editing
        • RNA & DNA Synthetic Standards
        • In Vitro Synthesised mRNA Service
        • Vector Cloning Service
        • NanoString Service
        • Aptamer Design and Synthesis
        • Exosome Research Services
        • Stable Cell Lines
        • Cytometry Service
  • Support
    • Close
    • Contact Us
    • Product Citations
    • FAQ –
    • Ordering Information
    • Distributors
  • About Us
    • Close
    • Partners
    • Events
    • News
    • Careers
Account
Settings
Language
USD Store
  • GBP Store
  • EUR Store
  • CHF Store
  • Secondary USD Store
  • Home
  • Grow a Pair: Reconstituting Human Testes from iPSCs

Cutting edge technology for vascularized kidney organoids
February 17, 2023
Investigating Glycans – shedding new light on the ‘dark matter’ of biology
January 10, 2023

Grow a Pair: Reconstituting Human Testes from iPSCs

In an innovative study in 2020, researchers Young Sun Hwang, Shinnosuke Suzuki, Yasunari Seita, and colleagues from the Sasaki lab devised a protocol in which induced pluripotent stem cells (iPSCs) could be utilised to produce reconstituted human testes. This exciting development allowed the Sasaki lab to recapitulate human male germ cell development in vitro, exposing the cellular mechanisms that occur in the foetal and early postnatal stages of life.

Non-obstructive azoospermia (NOA) is characterised by the failure of spermatogenesis and affects 10-15% of infertile men. Hwang and colleagues’ research brings us closer to understanding the processes that result in NOA by modelling prospermatogonial specification from iPSCs. Their novel study reliably and accurately produced human prospermatagonia in vitro and observed the expression of transposable elements that indicate development. The strides made by researchers in the Sasaki lab have paved a new way for future studies to explore treatments for male infertility.

StemFit (available from AMSBIO) was used in this study to maintain iPSC culture. StemFit is a range of feeder-free, weekend-free and chemically defined stem cell culture media suitable for basic and clinical research. The cells were cultured on plates coated in recombinant laminin E8 fragments using iMatrix-511 silk (available from AMSBIO). iMatrix-511 has become the gold standard for the maintenance and expansion of stem cell culture, as demonstrated by Hwang et al. in their protocol. The Sasaki lab also cryopreserved their cells using CELLBANKER 1 from AMSBIO, a trusted solution for the storage of any cell type including sensitive cell lines. CELLOTION™ cell wash and recovery solution from AMSBIO was also used by the lab to collect FACS-sorted in vitro cells prior to single-cell RNA-seq library preparation.

Figure 1. Immunofluorescent images of d14 xenogeneic reconstituted testes for germ cell markers (red: TFAP2C, DDX4, SOX17, POU5F1 or NANOG), markers for human PGCLC-derived cells (green: GFP; cyan: hMito), a Sertoli cell marker (cyan:SOX9), and DAPI (white), with their merges. Scale bars, 20 µm. (Licensed under a Creative Commons
Attribution 4.0 International License)

The gene regulatory networks affecting germ cell development amongst mammals are highly diverse and integral to the health and prosperity of a species. In 2022, researchers from the Sasaki lab used the same iPSC line and stem cell culture protocol established in the previous study again, utilising StemFit and iMatrix-511 silk, to maintain and grow their iPSC cells. This study by Jumpei Ito and colleagues characterised the transcriptomes of naïve pluripotent stem cells and primordial germ cell -like cells (PGCLCs), which highlighted a common gene regulatory network between the two cell types. These findings indicate a group of hominid-specific endogenous retroviruses, LTR5_Hs, are responsible for similarities in gene expression between iPSCs and PGCLCs. Ito and colleagues suggest that the insertion of LTR5_Hs was key during evolution, setting in motion the genetic changes that separated primates from other mammals.

AMSBIO is the global supplier of StemFit, iMatrix-511 and CELLBANKER. Our Stem Cell Synergy Solution offers a streamlined and efficient way to propagate, maintain, and store your stem cells, for basic research through to clinical applications. For quick and simple long-term storage of your cells, we recommend STEM-CELLBANKER cryopreservation medium to ensure high cell-viability post-thaw. Available in GMP-grade formulations, STEM-CELLBANKER retains cell identity and allows for direct freezing at -80°C. Find out more about how Stem Cell Synergy Solution could improve your research here.

References

  • Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells

Hwang, Y.S. et al. Nature communications, 2020, 11(1), 5656.

  • A hominoid-specific endogenous retrovirus may have rewired the gene regulatory network shared between primordial germ cells and naïve pluripotent cells.

Ito, J. et al. PLoS Genet, 2022, 18(5): e1009846.

Stem Cell Synergy Citations

  • Modelling SARS-CoV-2 infection and its individual differences with ACE2-expressing human iPS cells. 

Sano, E. et al. iScience, 2021, 24(5), 102428-102428

  • Efficient Adhesion Culture of Human Pluripotent Stem Cells Using Laminin Fragments in an Uncoated Manner.

Miyazaki, T. et al. Scientific reports, 2017, 7, 41165

  • High-Yield Human Induced Pluripotent Stem Cell-Derived Monocytes and Macrophages Are Functionally Comparable With Primary Cells

Cui, D. et al. Frontiers in cell and developmental biology, 2021, 9, 656867-656867

CELLBANKER® Cell Freezing Media

iMatrix Recombinant Laminin E8 fragments

Stem Cell Synergy Solution

STEM-CELLBANKER® Stem Cell Freezing Media

StemFit® Feeder-Free Stem Cell Culture Media

Need Help Finding a Product?

Our reagent specialists are to help you find the best product for your application. Please Call or Email us and we will be happy to help you find the right product for the job.

Call us at

+41 (0) 91 604 55 22

+49 (0) 69 779099

+44 (0) 1235 828 200

+31 (0) 72 8080244

+1 (617) 945 5033

Email Us

Subscribe to our newsletter

Be the first to hear about upcoming events, product news, special offers, application notes, and more

  • This field is for validation purposes and should be left unchanged.

Products

  • Biorepository
  • Molecular Biology
  • Antibodies
  • Proteins & Peptides
  • Cells & Cell Culture
  • Reagents & Labware
  • Kits & Assays
  • Bioseparation & Electrophoresis

Custom Services

  • Protein Expression
  • Lentivirus & Adenovirus
    & AAV
  • Peptide & Antibody
  • Tissue Procurement
  • Histochemistry
  • Stem Cell Differentiation
  • Cell Based Assays
  • Drug Screening Service
  • CAR-T Development
  • Molecular Biology
  • Magnetic Beads

Support

  • Product Citations
  • Contact Us
  • FAQ
  • Ordering Information
  • Distributors

About Us

  • Partners
  • Events
  • News
  • Careers

AMSBIO is a leading provider of quality life science research reagents and services.

Reg. in England and Wales no. 02117791.
ISO 9001 registered firm. Ⓒ2023

EORI Number: GB502237295000

Website Terms of Use - Terms of Sale - Privacy Policy Modern Slavery Statement