NEUROD1 Rabbit Polyclonal Antibody
Rabbit polyclonal NeuroD1 Antibody (N-term)
WB, IHC, IF
BETA2, BHF-1, bHLHa3, MODY6, NEUROD
Druggable Genome, Transcription Factors, ES Cell Differentiation/IPS, Adult stem cells, Embryonic stem cells
Western blot analysis of hNeuroD1-Q30 (Cat. #TA324696) in HepG2 cell line lysates (35ug/lane). NEUROD1 (arrow) was detected using the purified Pab.
IF image of HepG2 cell stained with hNeuroD1-Q30 (Cat#TA324696). HepG2 cells were incubated with hNeuroD1-Q30 primary antibody (1:25, 1 h at 37¶øC). For secondary antibody, Alexa Fluor¶© 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor¶© 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).hNeuroD1-Q30 immunoreactivity is localized to vesicles significantly.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.