HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEU2 (RC219858, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEU2. Positive lysates LY401652 (100ug) and LC401652 (20ug) can be purchased separately from OriGene.
Anti-NEU2 mouse monoclonal antibody (TA502942) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEU2 (RC219858 ).
Flow cytometric Analysis of Hela cells, using anti-NEU2 antibody (TA502942), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).