HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFA7 (RC200534, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFA7. Positive lysates LY417603 (100ug) and LC417603 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC200534 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NDUFA7 antibody (TA502702), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-NDUFA7 antibody (TA502702), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).