Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MSI1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-MSI1 mouse monoclonal antibody (TA502253) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MSI1 (RC215992).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSI1 (RC215992, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSI1. Positive lysates LY419331 (100ug) and LC419331 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MSI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502253)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MSI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502253)
HEK293T cells transfected with either RC215992 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MSI1 antibody (TA502253), and then analyzed by flow cytometry.