HEK293T cells transfected with either RC215992 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MSI1 antibody (TA502311), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSI1 (RC215992, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSI1. Positive lysates LY419331 (100ug) and LC419331 (20ug) can be purchased separately from OriGene.
Anti-MSI1 mouse monoclonal antibody (TA502311) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MSI1 (RC215992).