Mouse PD-L1 / TCR Activator Mammalian Expression Kit

Product Code
79778
$0.00
Order Support
Shipping Information
Product Updates
  • Keep up to date with product offers, resources and news

  • Sign up

The recombinant expression vectors are designed to express human engineered T cell receptor (TCR) activator and mouse PD-L1 (GenBank Accession #NM_021893) in mammalian cells. The transfected cells can be used in conjunction with mouse PD-1/NFAT Reporter/Jurkat T cells ( #79762) to study the interactions of PD-1 with PD-L1 ligand in a cellular context and to screen for modulators of this signaling pathway.Functional Validation and Assay Performance: In this assay/ mouse PD-1/NFAT Reporter/Jurkat T cells are used as effector cells; HEK293 cells transiently transfected with the PD-L1 vector over-express mouse PD-L1 and an engineered T cell receptor (TCR) activator and are used as target cells. When these two cells are co-cultivated/ TCR complexes on effector cells are activated by TCR activator on target cells/ resulting in expression of the NFAT luciferase reporter. However/ PD1 and PD-L1 ligation prevents TCR activation and suppresses the NFAT-responsive luciferase activity. This inhibition can be specifically reversed by anti-PD1 or anti-PD-L1 antibodies. Mouse PD1/PD-L1 neutralizing antibodies block PD1:PD-L1 interaction and promote T cell activation/ resulting in reactivation of the NFAT responsive luciferase reporter.
More Information
SKU 79778
Size 500 reactions
Alternate SKU AMS.79778
Species Mouse
Application • Screen for activators or inhibitors of mouse PD-1 signaling in a cellular context
• Characterize the biological activity of mouse PD-1 and its interactions with ligands
Gene synonyms Programmed Cell Death 1, PDCD1, SLEB2, CD279
Storage Store at -20C. Avoid freeze/thaw cycles. Stable at least 12 months from date of receipt/ when stored as directed.
Shipping Temp -20C
Supplier Name BPS