Mouse PD-L1 / TCR Activator Mammalian Expression Kit
Product Code
79778
$0.00
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The recombinant expression vectors are designed to express human engineered T cell receptor (TCR) activator and mouse PD-L1 (GenBank Accession #NM_021893) in mammalian cells. The transfected cells can be used in conjunction with mouse PD-1/NFAT Reporter/Jurkat T cells ( #79762) to study the interactions of PD-1 with PD-L1 ligand in a cellular context and to screen for modulators of this signaling pathway.Functional Validation and Assay Performance: In this assay/ mouse PD-1/NFAT Reporter/Jurkat T cells are used as effector cells; HEK293 cells transiently transfected with the PD-L1 vector over-express mouse PD-L1 and an engineered T cell receptor (TCR) activator and are used as target cells. When these two cells are co-cultivated/ TCR complexes on effector cells are activated by TCR activator on target cells/ resulting in expression of the NFAT luciferase reporter. However/ PD1 and PD-L1 ligation prevents TCR activation and suppresses the NFAT-responsive luciferase activity. This inhibition can be specifically reversed by anti-PD1 or anti-PD-L1 antibodies. Mouse PD1/PD-L1 neutralizing antibodies block PD1:PD-L1 interaction and promote T cell activation/ resulting in reactivation of the NFAT responsive luciferase reporter.
SKU | 79778 |
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Size | 500 reactions |
Alternate SKU | AMS.79778 |
Species | Mouse |
Application | • Screen for activators or inhibitors of mouse PD-1 signaling in a cellular context• Characterize the biological activity of mouse PD-1 and its interactions with ligands |
Gene synonyms | Programmed Cell Death 1, PDCD1, SLEB2, CD279 |
Storage | Store at -20C. Avoid freeze/thaw cycles. Stable at least 12 months from date of receipt/ when stored as directed. |
Shipping Temp | -20C |
Supplier Name | BPS |