IF image of NIH/3T3 cell stained with MOB4A Antibody (C-term) (Cat#TA324899).NIH/3T3 cells were incubated with MOB4A primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml).MOB4A immunoreactivity is localized to Cytoplasm significantly.
Formalin-fixed and paraffin-embedded human brain tissue reacted with MOB4A antibody (C-term) (Cat.#TA324899), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of lysates from K562, Daudi cell line (from left to right), using MOB4A Antibody (C-term) (Cat. #TA324899). TA324899 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
MOB4A Antibody (C-term) (Cat.# TA324899) western blot analysis in Daudi, K562 cell line lysates (35ug/lane).This demonstrates the MOB4A antibody detected the MOB4A protein (arrow).