Flow cytometric Analysis of Jurkat cells, using anti-MIOX antibody (TA501454), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIOX (RC210070, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIOX. Positive lysates LY413679 (100ug) and LC413679 (20ug) can be purchased separately from OriGene.
Anti-MIOX mouse monoclonal antibody (TA501454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MIOX (RC210070).
HEK293T cells transfected with either RC210070 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MIOX antibody (TA501454), and then analyzed by flow cytometry.