- High level expression system.
- Competitive price.
- Human cell line origin with full glycosylation.
- Scale up to 500 litres.
- Tag removal as requested
PHASE 1 : Cloning of your gene of interest into a vector with His-tag or IgG-Fc tag.
- Sequencing confirmation of insert.
- Small scale (5ml) transfection to test the expression level and optimization.
- Estimated time: 3-4 weeks
PHASE 2 : Large-scale transfection (500ml) of recombinant vector into HEK293 and protein expression, evaluation and optimization.
- One litre of bacterial culture will be induced with IPTG and harvested for protein purification.
- Protien purification from 500ml of HEK293 culture using affinity column of Ni-NTA or protein A (no tag cleavage service provided).
- The yield ranks from 3-25mg per 500ml with an average of 5mg.
- Estimated time: 2-3 weeks.
Deliverables from phase 2:
Protein and report including sequence confirmation, Western blot and SDS-PAGE result and design form.