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MEK4 (MAP2K4) Mouse Monoclonal Antibody [Clone ID: OTI1D6]

Product Code
TA500428
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$455.00

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anti-MAP2K4 (MEK4) mouse monoclonal antibody, clone OTI1D6 (formerly 1D6)
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Key Specs
SKU TA500428
Size 100 ul
Host species Mouse
Gene symbol MAP2K4
isotype IgG, IgG2, IgG2a
Application WB, IHC, IF, IP
Species reactivity Human
Accession No NM_003010
Gene id 6416
Gene synonyms JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1
Protein Families Druggable Genome, Protein Kinase
Storage -80C
Shipping Temp Ice Pack
Lead Time In Stock
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500428)
Anti-MAP2K4 mouse monoclonal antibody (TA500428) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4 (RC206051).
Immunoprecipitation (IP) of MAP2K4 by using TrueMab monoclonal anti-MAP2K4 antibodies (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500428)
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500428)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500428)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500428)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500428)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (RC206051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4. Positive lysates LY401058 (100ug) and LC401058 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500428)
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500428)

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