Anti-MAP2K1 mouse monoclonal antibody (TA500644) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K1 (RC218460).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K1 (RC218460, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1. Positive lysates LY400974 (100ug) and LC400974 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and MAP2K1-Knockout Hela cells (KO, Cat# LC810188) were separated by SDS-PAGE and immunoblotted with anti-MAP2K1 monoclonal antibody TA500644. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).