MEF2C Rabbit Polyclonal Antibody
Rabbit polyclonal MEF2C Antibody (S387)
WB, IHC, IF
Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C Antibody (S387), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Fluorescent confocal image of Hela cell stained with MEF2C Antibody (S387).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEF2C primary antibody (1:25, 1 h at 37¶øC). For secondary antibody, Alexa Fluor¶© 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37¶øC).Cytoplasmic actin was counterstained with Alexa Fluor¶© 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37¶øC). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MEF2C immunoreactivity is localized to vesicles and Nucleus significantly.
MEF2C Antibody (pS387) western blot analysis in HL-60, K562 cell line lysates (35ug/lane).This demonstrates the MEF2C antibody detected the MEF2C protein (arrow).