Determination of the titer.To determine the titer, an ELISA was performed using a serial dilution of the antibody against human MeCP2, in antigen coated wells. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:25,000.
WB analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MeCP2 (lane 2), diluted 1:2,000 in TBST containing 3% milk powder.Figure 2B: WB was performed on nuclear extracts from the human leukemic monocyte lymphoma cell line U937 (60 ug) with the antibody against human MeCP2, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.