HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (RC200521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1. P
Anti-MCL1 mouse monoclonal antibody (TA500990) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1 (RC200521).
HEK293T cells transfected with either pCMV6-ENTRY MCL1 (RC200521) (Red) or empty vector control plasmid (Blue) were immunostained with anti-MCL1 mouse monoclonal (TA500990), and then analyzed by flow cytometry.
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-MCL1 monoclonal antibody at 1:200 dilution.