MagSi-NA Pathogens Kit

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In the current status of the Covid-19 pandemic (high levels of testing; low infection prevalence) sample screening becomes less cost-effective. A solution to overcome this issue might be pooling and adding multiple samples together in one well and analyse them as one, after which only the positive wells’ samples need to be reanalysed. 

Unlike classic sample pooling methods, MagSi-NA Pathogens MSP enables the pooling of samples in a sequential and non-dilutive manner to save money on extractions and PCR reactions without compromising test sensitivity and resulting in false negatives.  

• MSP saves up to 80% in extraction and PCR reagents costs 
• MSP does not lower the sensitivity of your tests 
• MSP is easily automated on a PurePrep instrument 
• Pooling ratios of up to 6:1 possible 

Up to 6 times 96 samples are added to the wells of up to 6 deepwell plates. First, all samples are lysed under denaturing conditions by addition of lysis buffer including proteinase K and Poly-A-RNA. Magnetic MSP beads and binding buffer are then added to every sample

During the isolation procedure the magnetic MSP beads will bind the nucleic acids from all (up to 6) samples by transferring them in a serial manner to the next sample plate, until all (up to 6) samples have been incubated with magnetic MSP beads. 

After this, the magnetic MSP beads are washed in 3 deepwell plates containing alcoholic buffers. Finally, the nucleic acids are collected from the sample plates and released into an elution plate using a low-salt elution buffer.  

They can then be directly used for downstream applications 

Fig.1 Four concentrations of MS2 RNA were spiked at different positions in sample plates 1, 4 and 6 and used for the extraction protocol. qPCR results generated with the eluted RNA samples demonstrate  that there is no significant difference in RNA recovery between the binding steps of MagSi-NA Pathogens MSP, and compared to the standard MagSi-NA Pathogens procedures without pooling.

Fig 2. Ct values obtained by RT-qPCR from a COVID-19 positive saliva sample after RNA extraction without pooling and with MSP. The same sample was added to the first and last sample plates to evaluate sensitivity. No difference could be determined.