MagSi Magnetic Beads for Genomics & DNA Purification

Achieve efficient cost-effective separation of biomolecules

Magnetic beads with functionalized surfaces are a powerful tool used in a variety of research applications. AMSBIO supplies a unique range of magnetic silica beads for life science and bioanalytical processes. Our MagSi magnetic beads range in size and can be customized with a wide range of surface properties, functional groups and magnetic properties.

MagSi beads are specifically designed for the efficient and cost-effective separation, concentration and purification of biomolecules like proteins, DNA or RNA. We also offer beads for NGS (PCR cleanup and size selection) as well as for Sanger sequencing (dye terminator removal).

Benefits

  • High customization potential (e.g. sizes, surfaces, colors)
  • Speed and robustness
  • Flexibility and scalability
  • Ideal for automated systems
  • High binding capacity
  • Proven for downstream applications such as PCR etc.
  • Minimal non-specific absorption

Beads for Genomic Applications

AMSBIO offers a range of magnetic beads and kits specifically for use in genomic applications. Magnetic beads can be used as solid support phase in DNA extraction and purification protocols by a simple bind/wash/elute principle. Selecting the correct magnetic bead for your application is crucial to ensure you obtain the best possible results.

Silica beads

Intended for nucleic acid isolation from various sources (blood, cells, bacteria etc.) for manual and automated workflow.

Ultra-fast ferromagnetic silica particles with instant magnetic separation

Optimized magnetic content for fast separation and medium suspension time

Ferrimagnetic silica beads, developed for use in microfluidic and chip-based genomic setups

Large surface area and optimized magnetic content for long suspension time.

Very fast separation and shorter suspension time

Physical properties

MagSi-DNAMagSi-DNA 600MagSi-DNA allroundMagSi-DNA 3.0
Collection speed++++++++++
Time in suspension+/-++++
Accessible surface area+++++++
Bead density++++++++
AppearanceBlackBrownBrownBrown
Recommended preparation scaleAllMini/midiAllAll

Specifications

MagSi-DNAMagSi-DNA 600MagSi-DNA allroundMagSi-DNA 3.0
Mean diameter300nm600nm1.2µm3.0µm
Concentration300 mg/mL20 mg/mL20 mg/mL20 mg/mL
ShapeSphericalSphericalSphericalSpherical
Density3.1 g/cm31.2g/cm31.6g/cm31.6g/cm3
Surface area6.5 m2/g8.3 m2/g3.6 m2/g0.8 m2/g
DNA yield/isolation (20µl bead suspension)Up to 8 µgUp to 10 µgUp to 8 µgUp to 6 µg
DNA binding capacity~1.3 µg/mg~25 µg/mg~20 µg/mg~15 µg/mg
StabilitypH 3-11pH 3-11pH 3-11pH 3-11

Carboxylated Silica Beads

If a very high yield of DNA is needed, the MagSi-DNA beads are also available in carboxylated forms. Under specific conditions, the carboxylated surface enables higher yield and purity from samples.

Ultra-fast ferromagnetic silica particles with instant magnetic separation

Optimized magnetic content for fast separation and medium suspension time

Large surface area and optimized magnetic content for long suspension time.

Very fast separation and shorter suspension time

MagSi-DNA Trial Kit

A complete set of 8 types of MagSi beads for genomic applications, offered in a single kit for trial purposes in development of new extraction and purification protocols or replacement in existing protocols. Included in the kit are: silica beads MagSi-DNA, MagSi-DNA 600, MagSi-DNA allround, MagSi-DNA 3.0 and carboxylated beads MagSi-DNA COOH, MagSi-DNA 600 COOH, MagSi-DNA allround COOH, MagSi-DNA 3.0 COOH.

DNA Extraction Kits

For the extraction of genomic DNA from whole blood, saliva or vegetal material

MagSi-gDNA blood

For the extraction of genomic DNA from whole blood (fresh or frozen, EDTA or citrate) or buffy coat. Typical yield is 4-12 µg from 200 µL whole blood from an elution volume of 50-200 µL. The kit includes magnetic beads, buffers and Proteinase K, and is easy to automate for high-throughput processing. DNA is suitable for use in downstream applications such as PCR, genetic typing, SNP and mutation analysis and forensic analysis.

MagSi-gDNA saliva

Magnetic bead-based kit for extraction of DNA from human saliva or buccal swabs. The kit includes magnetic beads, buffers and Proteinase K, and is easy to automate for high throughput processing. DNA is suitable for use in downstream applications such as PCR, genetic typing, SNP and mutation analysis and forensic analysis.

MagSi-gDNA vegetal

For the extraction of genomic DNA from plant tissue. The kit has a very flexible setup as the volume of each of its components can be adjusted to facilitate your specific extraction protocol requirements

Kits for Purification of Sequencing & PCR Reactions

MagSi-DT Removal

An efficient solution for Dye-Terminator removal from BigDye® sequencing reactions

  • Optimized for use on Biomek® Laboratory Automation Workstations and Hamilton® Microlab® STARLine
  • Removes post-cycle sequencing reaction contaminants that interfere with sequencing analysis (in particular unincorporated dyes), without centrifugation or filtration
  • Can be used in high-throughput processes with 96 and 384 well plates.

MagSi-NGS PREP Plus

A convenient tool for ultra-fast and efficient purification and size selection of DNA products

  • Optimized for use on Biomek® Laboratory Automation Workstations and Hamilton® Microlab STAR™line
  • Allows for either non-selective binding, or size-targeted binding of double-stranded DNA fragments ranging from 80-1000bp with specific reagent volume to sample volume ratio’s (increasing the volume of MagSi-NGSPREP Plus, increases the efficiency of binding smaller fragments) This enables the user to selectively keep or discard undesired fragment sizes
  • Flexible protocols are easy to automate for high-throughput processing