HEK293T cells transfected with either RC205452 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LRRC50 antibody (TA504546), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-LRRC50 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LRRC50 (RC205452, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LRRC50. Positive lysates LY405926 (100ug) and LC405926 (20ug) can be purchased separately from OriGene.