HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2B3 (RC218359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2B3. Positive lysates LY401678 (100ug) and LC401678 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC218359 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SH2B3 antibody (TA502933), and then analyzed by flow cytometry.
Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-SH2B3 monoclonal antibody (1:200).
Flow cytometric Analysis of Jurkat cells, using anti-SH2B3 antibody (TA502933), (Red), compared to a nonspecific negative control antibody, (Blue).
Anti-SH2B3 mouse monoclonal antibody (TA502933) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2B3 (RC218359 ).
Flow cytometric Analysis of Hela cells, using anti-SH2B3 antibody (TA502933), (Red), compared to a nonspecific negative control antibody, (Blue).