HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (RC200062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1. Positive lysates LY402349 (100ug) and LC402349 (20ug) can be purchased separately from OriGene.
Anti-LMCD1 mouse monoclonal antibody (TA502790) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMCD1 (RC200062 ).
HEK293T cells transfected with either RC200062 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody (TA502790), and then analyzed by flow cytometry.