HEK293T cells transfected with either RC200062 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMCD1 antibody (TA502789), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMCD1 (RC200062, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMCD1. Positive lysates LY402349 (100ug) and LC402349 (20ug) can be purchased separately from OriGene.
Flow cytometric Analysis of Hela cells, using anti-LMCD1 antibody (TA502789), (Red), compared to a nonspecific negative control antibody, (Blue).