HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (RC207088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1. Positive lysates LY401709 (100ug) and LC401709 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and LMAN1-Knockout 293T cells (KO, Cat# LC812175) were separated by SDS-PAGE and immunoblotted with anti-LMAN1 monoclonal antibody TA502110, (1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Anti-LMAN1 mouse monoclonal antibody (TA502110) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1 (RC207088).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.