Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-BIRC7 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Flow cytometric analysis of Jurkat cells, using anti-BIRC7 antibody (TA500758), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC7 (RC204906, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC7. Positive lysates LY403386 (100ug) and LC403386 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-BIRC7 monoclonal antibody (1:200).
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BIRC7mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500758, Dilution 1:50)
Flow cytometric analysis of Hela cells, using anti-BIRC7 antibody (TA500758), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).