HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (RC209248, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG. Positive lysates LY401821 (100ug) and LC401821 (20ug) can be purchased separately from OriGene.
Immunoprecipitation (IP) of LIPG by using TrueMab monoclonal anti-LIPG antibodies (Negative control: IP without adding anti-LIPG antibody.). For each experiment, 500ul of DDK tagged LIPG overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-LIPG antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Anti-LIPG mouse monoclonal antibody (TA501058) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIPG (RC209248).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LIPG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501058)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-LIPG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501058)