Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LIPG monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LIPG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501001)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIPG (RC209248, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIPG. P
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LIPG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501001)
Immunoprecipitation (IP) of LIPG by using TrueMab monoclonal anti-LIPG antibodies (Negative control: IP without adding anti-LIPG antibody.). For each experiment, 500ul of DDK tagged LIPG overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of