HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIN7B (RC205598, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIN7B. Positive lysates LY411720 (100ug) and LC411720 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC205598 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIN7B antibody (TA501480), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-LIN7B antibody (TA501480), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).