HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIN7B (RC205598, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIN7B. Positive lysates LY411720 (100ug) and LC411720 (20ug) can be purchased separately from OriGene.
Anti-LIN7B mouse monoclonal antibody (TA501555) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIN7B (RC205598).
HEK293T cells transfected with either RC205598 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIN7B antibody (TA501555), and then analyzed by flow cytometry.