LIN28A Antibody (Center) (Cat. #TA324388) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
LIN28A Antibody (Center) (Cat. #TA324388)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LIN28A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
LIN28A Antibody (Center) (Cat. #TA324388) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the LIN28A antibody detected the LIN28A protein (arrow).
IF image of SY5Y cells stained with TA324388 LIN28A (Center) antibody. SY5Y cells were incubated with TA324388 LIN28A (Center) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin. Lin28a immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
Immunofluorescent analysis of A549 cells, using LIN28A Antibody (Center) (Cat. #TA324388). TA324388 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight FluorÃ‚Â® 554 (red) conjugated Phalloidin (red).