Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-LGALS3BP monoclonal antibody (1:200).
Anti-LGALS3BP mouse monoclonal antibody (TA503454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LGALS3BP (RC204918).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGALS3BP (RC204918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGALS3BP. Positive lysates LY417206 (100ug) and LC417206 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC204918 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LGALS3BP antibody (TA503454), and then analyzed by flow cytometry.