HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LENG1 (RC204931, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LENG1. Positive lysates LY411314 (100ug) and LC411314 (20ug) can be purchased separately from OriGene.
Anti-LENG1 mouse monoclonal antibody (TA503083) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LENG1 (RC204931).
HEK293T cells transfected with either RC204931 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LENG1 antibody (TA503083), and then analyzed by flow cytometry.