Anti-SLC7A8 mouse monoclonal antibody (TA500503) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC7A8 (RC208586).
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SLC7A8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500503)
HEK293T cells transfected with either RC208586 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLC7A8 antibody (TA500503), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (RC208586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8. Positive lysates LY402177 (100ug) and LC402177 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SLC7A8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Immunoprecipitation (IP) of SLC7A8 by using TrueMab monoclonal anti-SLC7A8 antibodies (Negative control: IP without adding anti-SLC7A8 antibody.). For each experiment, 500ul of DDK tagged SLC7A8 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC7A8 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.