HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (RC208586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC7A8. Positive lysates LY402177 (100ug) and LC402177 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC208586 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLC7A8 antibody (TA500514), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SLC7A8 monoclonal antibody.
Anti-SLC7A8 mouse monoclonal antibody (TA500514) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SLC7A8 (RC208586).