Western blot analysis of extracts (35ug) from 4 cell line lysates by using anti-L1CAM monoclonal antibody (1:500).
Flow cytometric analysis of living Hela cells, using anti-L1CAM antibody (TA501441, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).
Anti-L1CAM mouse monoclonal antibody (TA501441) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM (RC211601).
Flow cytometric analysis of living A375 cells, using anti-L1CAM antibody (TA501441, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM. Positive lysates LY400150 (100ug) and LC400150 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC211601 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody (TA501441), and then analyzed by flow cytometry.