Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
HEK293T cells transfected with either RC211601 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody (TA501438), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM. Positive lysates LY400150 (100ug) and LC400150 (20ug) can be purchased separately from OriGene.
Flow cytometric Analysis of Hela cells, using anti-L1CAM antibody (TA501438), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).