IF image of SY5Y cells stained with TA324725 KLF4 (S457) antibody. SY5Y cells were incubated with TA324725 KLF4 (S457) primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin. Note the highly specific localization of the phosphorylated KLF4 immunoreactivity to the nuclei.
Western blot analysis of KLF4-S457 (Cat.# TA324725) in MDA-MB231, NCI-H460 cell line lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.