IF image of SY5Y cells stained with TA324452 KLF4 (C-term) antibody. SY5Y cells were incubated with TA324452 KLF4 (C-term) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin. KLF4 immunoreactivity is localized very specifically to the nuclei of the SY5Y cells.
KLF4 Antibody (C-term) (Cat. #TA324452) western blot analysis in A431, MCF-7 cell line lysates (35ug/lane).This demonstrates the KLF4 antibody detected the KLF4 protein (arrow).
KLF4 Antibody (C-term) (Cat. #TA324452) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.