HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FERMT2 (RC205727, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FERMT2. Positive lysates LY416377 (100ug) and LC416377 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-FERMT2 monoclonal antibody (1:200).
Immunofluorescent staining of HeLa cells using anti-FERMT2 mouse monoclonal antibody (TA500509).
Anti-FERMT2 mouse monoclonal antibody (TA500509) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2 (RC205727).
Immunoprecipitation of FERMT2 by using TrueMab monoclonal anti-FERMT2 antibodies (Negative control: IP without adding anti-FERMT2 antibody.). For each experiment, 500ul of DDK tagged FERMT2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-FERMT2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.