HEK293T cells transfected with either RC202424 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody (TA501247), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (RC202424, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK. Positive lysates LY400082 (100ug) and LC400082 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501247, Dilution 1:50)