HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KEAP1 (RC202189, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 (1:1000).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and KEAP1-Knockout Hela cells (KO, Cat# LC810295) were separated by SDS-PAGE and immunoblotted with anti-KEAP1 monoclonal antibody TA502056. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).
HEK293T cells transfected with either RC202189 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KEAP1 antibody (TA502056), and then analyzed by flow cytometry.
Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY KEAP1 (RC202189) using anti-KEAP1 antibody (TA502056/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).