ISRE Luciferase Reporter Lentivirus (JAK/STAT Signaling Pathway)

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AMS.79824
$0.00
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The JAK/STAT pathway is activated by various cytokines and growth factors and plays a critical role in cell growth/ hematopoiesis/ and immune response. In mammals/ there are four JAKs (JAK1/ JAK2/ JAK3 and TYK2) and seven STAT proteins. IFN? is a Type I interferon. Binding of IFN? to its receptor leads to the activation of JAK1 and TYK2/ which in turn phosphorylate and activate STAT1 and STAT2. The phosphorylated STAT1 and 2 form a heterodimer and bind to IRF9/p48/ forming a protein complex ISGF3. This complex translocates to the nucleus and binds to the ISRE (Interferon Stimulated Response Element) in the promoter region/ thereby promoting transcription of interferon-inducible genes.
The ISRE Luciferase Reporter Lentivirus are replication incompetent/ HIV-based/ VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells/ including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by multimerized ISRE response element located upstream of the minimal TATA promoter. After transduction/ the activity of Type I interferon-induced JAK/STAT signaling pathway in the target cells can be monitored by measuring the luciferase activity.
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SKU AMS.79824
Size 500 ul x 2
Gene symbol JAK reporter, STAT reporter, ISRE reporter, IFN, lentivirus reporter,
Application • Screen for activators or inhibitors of Type I interferon induced JAK/STAT signaling pathway in the transduced target cells • Generation of ISRE Luciferase Reporter stable cell line
Gene synonyms JAK reporter, STAT reporter, ISRE reporter, IFN, lentivirus reporter,
Storage Lentiviruses are shipped with dry ice. For long term storage/ it is recommended to store the virus at -80C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Shipping Temp -80C (dry ice)
Supplier Name BPS