HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH1 (RC210582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1. Positive lysates LY401782 (100ug) and LC401782 (20ug) can be purchased separately from OriGene.
Anti-IDH1 mouse monoclonal antibody (TA500610) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH1 (RC210582).
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-IDH1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and IDH1-Knockout Hela cells (KO, Cat# LC810112) were separated by SDS-PAGE and immunoblotted with anti-IDH1 monoclonal antibody TA500610. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH1 monoclonal antibody.
Figure from citation: Western Blot of IDH1 protein level by using anti-IDH1 antibody in normal human astrocyte (NHA) cells. View Citation