HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (RC209951, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3 (1:500).
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-IRF3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY IRF3 (RC209951) using anti-IRF3 antibody (TA500476/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).
HEK293T cells transfected with either RC209951 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IRF3 antibody (TA500476), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100Â°C for 10min, TA500476)