Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and IRF3-Knockout HeLa cells (KO, Cat# LC833375) were separated by SDS-PAGE and immunoblotted with anti-IRF3 monoclonal antibody TA809334 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY IRF3 (RC209951) using anti-IRF3 antibody (TA809334/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (RC209951, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3 (1:500).
Western blot analysis of extracts (35ug) from 6 different cell lines and 2 tissue lysates by using anti-IRF3 monoclonal antibody (1:500).