Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500067)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 (RC202078, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6. Positive lysates LY424617 (100ug) and LC424617 (20ug) can be purchased separately from OriGene.
IL-6 antibody (3G9) at 1:500000 + recombinant human IL-6 (Cat# RC202078) IL-6 antibody (2G3) at 1:2000
Anti-IL-6 mouse monoclonal antibody (TA500067) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL-6 (RC202078).