Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.Antibody dilution:1:400. Image and statement courtesy of the Human Protein Atlas (HPA).
Western Blot: IGF2BP2 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil Target weight: 66, 62 (splice variants) Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
Immunofluorescent staining of human cell line U-251 MG shows positivity in cytoplasm. Antibody dilution: 1:100. Image and statement courtesy of the Human Protein Atlas (HPA).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (RC205673, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2. Positive lysates LY401961 (100ug) and LC401961 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).